Table 1.
Sample | IgA concentration (AU) | IgG concentration (AU) | IgA CV (%) | IgG CV (%) |
---|---|---|---|---|
Intra-assay variation | ||||
1 | 510·2 | 156·2 | 1·7 | 1·2 |
2 | 992·6 | 454·2 | 2·2 | 1·7 |
3 | 1169·4 | 1487·5 | 1·0 | 1·4 |
Interassay variation | ||||
4 | 129·5 | 847·2 | 3·9 | 2·3 |
5 | 170·8 | 423·3 | 3·1 | 3·5 |
6 | 224·7 | 278·1 | 3·1 | 3·6 |
The intra-assay variation was determined on three different peptide AGA IgA- and IgG-positive sera which were applied in quadruplicate at five different locations on a microtitre plate. The interassay variations were determined with three different peptide AGA IgA- and IgG-positive sera using triplicates for each sample on a microtitre plate in 10 identical assays on eight separate days.