Table 1.
Effects of immunoglobulin G (IgG) on the expression of cell surface molecules in healthy controls.
| Day 7 | Day 9 | ||||
|---|---|---|---|---|---|
| Molecule | Monocyte | IgG | Saline | IgG | Saline |
| CD1a | 6·9 ± 4·3 | 59·8 ± 12·0** | 74·5 ± 9·3 | 64·4 ± 8·5** | 79·5 ± 8·0 |
| CD83 | 3·7 ± 2·6 | 20·9 ± 8·7** | 14·3 ± 9·0 | 50·1 ± 6·6** | 29·7 ± 2·8 |
| CD40 | 3·9 ± 2·3 | 34·0 ± 9·7 | 34·4 ± 10·3 | 53·9 ± 13·0** | 81·0 ± 8·7 |
| CD80 | 0·8 ± 0·8 | 25·4 ± 8·2* | 32·3 ± 9·5 | 48·9 ± 14·1** | 81·4 ± 7·2 |
| CD86 | 78·0 ± 14·2 | 74·3 ± 13·2** | 51·5 ± 17·5 | 96·8 ± 3·0* | 84·7 ± 15·5 |
| HLA-DR | 46·0 ± 19·5 | 21·4 ± 11·9 | 21·1 ± 12·2 | 52·6 ± 24·0 | 48·1 ± 22·3 |
| CD49d | 94·4 ± 3·4 | 39·7 ± 8·2** | 29·5 ± 8·7 | 27·5 ± 10·9** | 49·8 ± 12·3 |
Monocyte isolation and differentiation into DCs were performed as described in Materials and methods. Healthy control samples (n = 8) were each divided into a group with IgG added at the beginning of the culture period (IgG) and a group treated with vehicle alone (saline). Monocytes and cells on days 7 and 9 of treatment were analysed by flow cytometry. The results show the frequency of cells (%) positive for each cell surface molecule [CD1a, CD83, CD40, CD80, CD86, human leucocyte antigen D-related (HLA-DR), and CD49d] as the mean value ± standard deviation. To calibrate the differences between the paired mean values, we used a paired t-test
(P < 0·01
P < 0·05).