Table 2.

Effects of immunoglobulin G (IgG) on the intracellular production of interleukin (IL)-10 and IL-12 associated with mature dendritic cell (mDC) differentiation in healthy controls.

	CD1a+		CD1a–
Cytokine	IgG	Saline	IgG	Saline
IL-12	18·1 ± 8·4**	31·6 ± 8·3	5·4 ± 2·9	5·5 ± 4·4
IL-10	15·1 ± 7·5	14·6 ± 6·5	11·9 ± 4·7*	7·4 ± 3·4

Samples from healthy controls (n = 10) were each divided into two groups, one with IgG added at the beginning of culture (IgG) and one treated with vehicle alone (saline). Immature DCs collected from the cultures on day 7 were resuspended in medium containing tumour necrosis factor (TNF)-α, IL-1β, and Golgi Stop. Cells were collected after 12 h. We measured cell surface molecules (CD1a) and intracellular cytokines (IL-12 and IL-10) as described in Materials and methods. The results indicate the frequency of positive cells (%) in each quadrant as the mean value ± standard deviation. To calibrate the differences between paired mean values, we used a paired t-test(

^**P < 0·01

^*P < 0·05).