Fig. 3.

One-way mixed lymphocyte reaction (MLR), assessing the inhibitory capacity of soluble cytotoxic T lymphocyte antigen 4 (CTLA4) proteins in an unprimed alloreactive setting. Stimulator cells were human leucocyte antigen (HLA) DR/DR 4/4, responder cells were D-related 3/3. The x-axis indicates the added concentration of protein, the y-axis indicates proliferation in counts per minute (cpm). ▪ = MLR without addition (positive control), □ = medium without cells (negative control), • = CTLA4Ig, ▴ = LEA29Y, ▾ = soluble CTLA4. Shown are mean ± standard error. These results were reproduced twice.