Fig. 2.

Phenotypic analysis of CD4⁺CD25^high T cells and forkhead box P3 (FoxP3) expression. (a) CD4⁺ cells were sorted in CD4⁺CD25^high and CD4⁺CD25⁻ and then stained with anti-CD25, anti-glucocorticoid-induced tumour necrosis factor receptor family-related protein (GITR) and anti-CD152 antibodies. CD4⁺CD25⁺ and CD4⁺CD25⁻ cell populations were analysed separately. (b) cDNA obtained from purified populations of CD4⁺CD25⁻ and CD4⁺CD25^high cells from 49 cancer patients and 24 healthy donors were subjected to quantitative real-time polymerase chain reaction (PCR) analysis using primers specific for FoxP3 and Abelson (Abl), as described in Materials and Methods.