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. Author manuscript; available in PMC: 2008 Jul 1.
Published in final edited form as: Brain Behav Immun. 2007 Feb 26;21(5):522–527. doi: 10.1016/j.bbi.2006.12.008

Figure 1. Confocal microscopy of myelinated teased fiber preparations from rat sciatic nerve.

Figure 1

Panel A shows TOPRO-3, a nuclear stain (blue), and the presence of one Schwann cell nucleus; Panel B shows a phase contrast image of the teased fiber; Panel C shows immunofluorescence with polyclonal antibody to a particularly interesting new cysteine histidine protein (PINCH) (Alexa 488, green); Panel D shows immunofluorescence using anti-myelin associated glycoprotein (MAG) (Alexa 564, red), a marker of Schwann cell cytoplasm where myelin is not compact; Panel E shows colocalization of MAG, PINCH and TOPRO-3. Note the peri-nuclear staining of PINCH and localization in compartments of non-compact myelin. Images represent at least 10 separate fibers analyzed. Scale bar 10 μm.