TABLE 2.
Phenotypes following shift-up of mpk-1(ga111) to 25°
| % hermaphrodite gonad arms with indicated phenotype
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Pachytene cellular organization
|
Proximal pachytene nuclei | Normal diakinetic nuclei | ||||||||
| Genotype | Gaps in sheet of cells | Internal cells/nuclei | Pycnotic nucleia | Disorganized oocytes | Emo | Normal sperm | Mitotic zone size | n | ||
| Embryo shift-up | ||||||||||
| Wild type | 0 | 0 | 0 | 0 | 100 | 0 | 0 | 100 | 18 ± 2.1 | 22 |
| mpk-1(ga111) | 97 | 41 | 84 | 91 | 19 | 9 | 16 | 97 | 18 ± 2.3 | 32 |
| L4 shift-up | ||||||||||
| Wild type | 0 | 0 | 0 | 0 | 100 | 0 | 0 | 100 | 19 ± 1.8 | 22 |
| mpk-1(ga111) | 94 | 6 | 0 | 66 | 66 | 16 | 41 | 97 | 19 ± 2.6 | 32 |
The temperature-sensitive mpk-1(ga111) is described in Lackner and Kim (1998). Animals were collected for gonad dissection 24 hr post L4 at 25°.
a
Pycnotic nuclei can contain HIM-3 aggregates.