TABLE 3.

Phenotypes of let-60(ga89gf)

	% hermaphrodite gonad arms with indicated phenotype
Oocyte phenotypes
Genotype	Disorganized	Small oocytes, multiple rows	Emo	Ectopic dpMPK-1	Mitotic zone sizec	n
20°a
Wild type	0	0	0	0	21 ± 1.8	20
let-60(ga89gf)	10	0	0	100	21 ± 1.6	30
25° shift-upb
Wild type	0	0	0	0	21 ± 1.6	20
let-60(ga89gf)	40	60	17	100	20 ± 1.5	30

let-60(ga89gf) is described in Eisenmann and Kim (1997). let-60(n1046gf) (Beitel et al. 1990; Han et al. 1990) hermaphrodites were also examined: First, mutant hermaphrodites are fertile at 15°, 20°, and 25°. At 20°, 58% of gonad arms have a mild disorganized oocyte phenotype (n = 31). At all temperatures, mutants lay small embryos. Time-lapse video analysis at 20° shows that the small embryos primarily arise from premature closure of the proximal gonad/distal spermathecal valve, resulting in the maturing oocyte being chopped during ovulation into the spermatheca. Second, the levels and spatial pattern of dpMPK-1 are similar in let-60(n1046gf) and wild type (see supplemental Figure 5 at http://www.genetics.org/supplemental/).

^aAnimals were picked at mid-L4 and 24 hr later gonads were obtained by dissection.

^bAnimals were picked at mid-L4, grown for an additional 20 hr at 20°, and shifted to 25° for 4 hr; then gonads were obtained by dissection.

^cLength of the mitotic zone, in cell diameters from the distal tip, on the basis of DAPI nuclear morphology.