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. Author manuscript; available in PMC: 2009 Jan 1.
Published in final edited form as: Neuropharmacology. 2007 Jun 6;54(1):1–7. doi: 10.1016/j.neuropharm.2007.05.020

Fig. 2.

Fig. 2

The effect of siRNA knockdown of NAPE-PLD, Abhd4 or PTPN22 on LPS-induced AEA synthesis in RAW264.7 cells. The degree of knockdown was verified by real-time PCR in mock-transfected (white columns) vs siRNA-transfected cells (shaded columns, left side). The right two pairs of columns indicate the cellular levels of AEA following vehicle (open columns) or LPS treatment (hatched columns) of mock- or siRNA transfected cells. Means ± SE from three experiments are shown. Significant difference from corresponding vehicle-treated group (*) or from corresponding mock-transfected group (#) is indicated, P < 0.05. Comparison of relative mRNA levels was analyzed by the paired t test, whereas the effect of LPS on cellular AEA levels was analyzed using the unpaired t test.