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. 2007 Jul 19;37(6):651–659. doi: 10.1165/rcmb.2006-0428OC

Figure 1.

Figure 1.

Integrin β6 subunit-deleted (Itgb6−/−) mice spontaneously develop elevated bronchoalveolar lavage (BAL) phospholipid and collectin levels associated with large foamy alveolar macrophages and inflammation. (a) Representative BAL fluid cytospins from Itgb6−/− mice revealed enlarged alveolar macrophages with cytoplasmic vacuolization and extracellular debris. (b) Representative high-power microscopic images of lung tissue showed enlarged size and numerous vacuoles in macrophages from Itgb6−/− mice; this was not observed in any of the sections from littermate controls. Arrows indicate alveolar macrophages. (c) PAS (left) and Oil red O (right) staining of inflated lung sections from Itgb6−/− mice demonstrate cytoplasmic staining (PAS stains glycoprotein pink; Oil red O stains lipid red). Arrows indicate alveolar macrophages. (d) Total phospholipids, SP-A, and SP-D in BAL fluid from wild-type (WT) littermates and Itgb6−/− mice expressed as mean ± SEM (n = 6 per group). Results are representative of three separate experiments; *P < 0.005. (e) BAL fluid cell counts and differentials in wild-type littermates and Itgb6−/− mice; * P < 0.008. Mice studied in b were 6 months old, while all other data were collected from 2-month-old mice. Scale bars indicate 20 μm.