Figure 2.

Specific binding of recombinant FimH, FimH-expressing bacteria, or CD48-specific antibody to CD48 in mast cell membrane preparations. Lanes: 1, MeαMan-eluted material from the Con A column after SDS/PAGE and Coomassie blue staining; 2 and 3, PVDF membrane blots of Con A-eluted material probed with ¹²⁵I-labeled FimH–FimC complex in the absence or presence, respectively, of 100 mM MeαMan; 4 and 5, PVDF membrane blots of Con A-eluted material probed with biotinylated FimH-expressing E. coli or biotinylated FimH-minus E. coli, respectively; 6, PVDF membrane blot of Con A-eluted material probed with biotinylated FimH-expressing E. coli in the presence of 100 mM MeαMan; 7, PVDF membrane blot of Con A-eluted material probed with rat CD48-specific mouse monoclonal antibody; 8, PVDF membrane blot of a whole cell lysate of BMMCs probed with rat monoclonal antibody to mouse CD48.