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. 1999 Jul 6;96(14):8110–8115. doi: 10.1073/pnas.96.14.8110

Figure 3.

Figure 3

Inhibition of mast cell association with FimH-expressing bacteria after pretreatment with increasing concentrations of PLC and antibody to CD48. (A) BMMC suspensions were pretreated with indicated concentrations of PLC for 1 hr and then PLC was removed. The PLC treated cells were seeded in wells of a 96-well tissue culture plate in the presence of 1 μM actinomycin D. After overnight incubation, bacterial adherence assays were carried out on these cells. (B) Monolayers of BMMCs in a 96-well tissue culture plate were coated with 1% human gamma globulin for 1 hr at 37°C. The monolayers were then incubated with indicated concentrations of antibody against either CD48 or CD117 (control) for 1 hr at 37°C. Bacterial adherence assays were then done, and the number of mast cell associated bacteria was determined by standard viable counts on agar plates. The data points represent the mean ± SEM values obtained from three experiments.