Figure 4.

Binding of FimH-expressing E. coli to processed and unprocessed recombinant CD48. Equal concentrations (5 μg) of processed (lanes 1 and 2), processed and deglycosylated (lane 3), and unprocessed (lane 4) soluble CD48 were subjected to SDS/PAGE and then transferred onto PVDF membranes. The corresponding strips were exposed for 1 hr to biotinylated FimH-expressing E. coli in the absence (lanes 1, 3, and 4) or presence (lane 2) of 100 mM MeαMan. Bound bacteria were detected by the addition of streptavidin-peroxidase and an appropriate substrate. Similar results were obtained in two experiments.