Figure 5.

Induced expression of CD48 on CHO cells increases binding of FimH-expressing E. coli. (A) Expression of CD48 on transfected CHO cells assessed by flow cytometry analysis. The open peak represents control transfected CHO cells (with cDNA encoding the C terminus of conglutinin) and the solid peak represents CHO cells transfected with cDNA encoding rat CD48. The cells were labeled with CD48-specific mouse monoclonal antibody and fluorescein isothiocyanate-labeled second antibody. Similar results were obtained in three experiments. (B) Binding of FimH-expressing bacteria to CD48-expressing CHO cells and control CHO cells. CHO-cell-associated bacteria were quantitated by standard viability assays. The results are expressed as percent of control. The data points represent the mean ± SEM values obtained from three experiments.