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. Author manuscript; available in PMC: 2008 Nov 1.
Published in final edited form as: Bone. 2007 Aug 11;41(5):752–759. doi: 10.1016/j.bone.2007.07.020

FIG. 4. Osteoblast populations in the PTHrP-lacZ mouse do not contain β gal activity.

FIG. 4

(A and B) CryoJane sections of day 12 (D12) proximal tibia stained with X-gal and counterstained with nuclear fast red (A, 100X) or with alkaline phosphatase (B, 100X) using a fast red violet LB salt that generates a red reaction product. The arrows in B identify the secondary ossification center, primary spongiosa, groove of Ranvier, and the bone collar, which is seen as a vertical strip of cells with a very high activity. It should be noted that hypertrophic chondrocytes also produce alkaline phosphatase, as seen in the population in the secondary ossification center just subjacent to the articular chondrocytes. (C) Proximal tibia at 6 weeks, X-gal-stained and counterstained with H & E (200X). (D) CryoJane section from 8-week-old PTHrP-lacZ mouse on a C57BL/6 background, stained and counterstained as in A. There is no β gal activity in the primary spongiosa in either panel C or D.