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. 1999 Jul 6;96(14):8120–8125. doi: 10.1073/pnas.96.14.8120

Figure 5.

Figure 5

Expression of Mena, Evl, profilin, zyxin, vinculin, and cGKI in platelets and selected tissues from wild-type, VASP+/−, and VASP−/− mice. (a) Immunoblot analysis. Whole-cell and tissue extracts (30 μg protein) were separated, blotted, and labeled as described in Materials and Methods. Equal protein loading was controled by Ponceau staining. Profilin, 12–15 kDa; zyxin, 83 kDa; vinculin, 117 kDa; cGKI, 74 kDa; Mena in brain, 88 kDa and 140 kDa; and Mena in lung, 80 kDa and 88 kDa. (b) Northern blot analysis of Evl expression. Poly(A)+ RNA (3 μg) were loaded per lane, as described in Materials and Methods. The blot was hybridized with a mouse Evl cDNA probe.