Table 5.
Real-time PCR primers for validation of microarray data.
| Affymetrix Probeset ID | Gene | Product size (bp) | Primers Forward Reverse |
| 1387128_at | Adcy3: adenylate cyclase 3 | 148 | agcacctatatggcagcttctgga |
| taagcgtgtccttcatggctagtg | |||
| 1370131_at | Cav1: caveolin 1 | 150 | gcttcaccaccttcactgtgacaa |
| ggaagctcttgatgcacggtacaa | |||
| 1367600_at | Des: desmin | 164 | gatcaaccttccgatccagacctt |
| gcacttcatgttgttgctgtgtgg | |||
| 1371166_at | NOS3: endothelial nitric oxide | 154 | tctggcaagaccgattacacgaca |
| synthase, eNOS | gtgaggacttgtccaaacactcca | ||
| 1386881_at | Igfbp3: insulin-like growth factor- | 167 | tggaaacaccactgagtctgagga |
| binding protein 3 | agtcaactttgtagcgctggctgt | ||
| 1373499_at | Gas5: growth arrest-specific 5 | 152 | ccacctgattcaaactccaccatc |
| tctgtgatgggacatctggtggaa | |||
| 1375857_at | Myof: myoferlin | 105 | cgctgccgaactatttatccatga |
| cagacgtatgtgaggcgagagttc | |||
| 1371776_at | Pik3r1: phosphatidylinositol 3- | 146 | ggcgaagtcaaacattgcgtcatc |
| kinase regulatory subunit polypeptide 1 | gtgacattgagggagtcattgtgc | ||
| 1387074_at | Rgs2: regulator of G-protein | 151 | ggtgtacagcctgatggagaacaa |
| signaling 2 | tgggagacagaatggaatgtcctc | ||
| 1370228_at | Tf: transferrin | 140 | cgaacggaaagaacactgctgcat |
| gaccacaacatggtttggagcttg | |||
| 1372002_at | Gja1: connexin43 Cx43 | 306 | gagatgcacctgaagcagattgaa |
| gatgttcaaagcgagagacaccaa | |||
| 1368473_at | Gja5: connexin40 Cx40 | 236 | ggaaagaggtgaacgggaagatt |
| cacagccatcataaagacaatgaa | |||
| 1370023_at | Gja4: connexin37 Cx37 | 131 | agctctgcatccaagaagcagta |
| agttgtctctcaagtgcctttga | |||
| - | 18S rRNA GenBank acc. no. | 110 | ccagtagcatatgcttgtctcaa |
| X01117 | cgaccaaaggaaccataactgatt |