FIGURE 6. Near UV CD.

Near UV CD spectra for E123CaG-2 (dashed line), E122′3CaG-4 Ala³⁸⁷ (solid line), and E122′3CaG-4 Pro³⁸⁷ (dotted line) are shown in the presence of 2 mm CaCl₂ (A) and upon removal of CaCl₂ by the addition of 2 mM EDTA (B). Spectra were measured at 25 °C in 10 mm MOPS, 150 mm NaCl, 0 –2 mm CaCl₂, pH 7.5. Representative spectra are shown.