Figure 2.

Production of CFC and HPP-CFC in LTBMC. At various times in culture, 2,000–5,000 NA cells were cultured and colonies were counted as described in Materials and Methods. The frequency of colonies obtained was multiplied by the number of NA cells per flask to obtain the number of CFC per flask. Total number of (A) CFC per flask and (B) HPP-CFC per flask.