Figure 3.
Competitive repopulation assays of culture-derived HSC. (A) Long-term repopulating HSC in 2-month-old TPO-LTBMC, but not control LTBMC. Lethally irradiated C57BL/6 (CD45.2) mice were transplanted with fresh CD45.1 bone marrow cells only, or with 5 × 105 CD45.2 bone marrow cells plus 5.5 × 105 or 1.34 × 106 CD45.1 NA cells from control or TPO-LTBMC. Bars indicate the percent CD45.1+ cells in peripheral blood at various times after transplant. (B) Limiting dilution analysis of cells from 3-month-old TPO-LTBMC. Lethally irradiated C57BL/6 mice were transplanted with 3 × 105 C57BL/6 bone marrow cells, and 2.5 × 104, 5.0 × 104, 1 × 105, or 2 × 105 NA cells from 3-month TPO-LTBMC. (C) HSC in NA cells from 4-month-old TPO-LTBMC. Lethally irradiated animals were transplanted with syngeneic normal bone marrow and 1 × 105 or 5 × 105 NA cells, and analyzed as described above.