Table 6.
Culture
|
Percent positive animals (no. positive/transplanted)
|
Repopulating units per 105 cells (95% confidence interval) | |||||
---|---|---|---|---|---|---|---|
Type | Time | 5,000 cells* | 10,000 cells* | 25,000 cells* | 50,000 cells* | 100,000 cells* | |
TPO | 1 month | 0 (0/6) | 0 (0/7) | 33 (2/6) | 75 (3/4) | 0.9 (0.1–1.7) | |
2 months | 13 (1/8) | 57 (5/7) | 100 (5/5) | 100 (5/5) | 6.7 (2.8–11.5) | ||
Control | 1 month | 13 (1/8) | 60 (3/5) | 1.6 (0.1–3.0) | |||
2 months | 0 (0/5) | 14 (1/7) | 33 (2/6) | 60 (3/5) | 0.9 (0.2–1.6) |
Animals were assayed 3 months posttransplant. Transplanted animals were considered positive if >0.5% of the peripheral leukocytes expressed CD45.1. Repopulating units were calculated by using the maximum-likelihood model (25).
Number of NA cells transplanted.