TABLE 1.
Branchiomotor Neurons Are Reduced in Number in mind bomb Mutants
| Islet antibody-labeled cell type | WT (ta52b)a | mibta52b | Ratio (mutant/WT) |
|---|---|---|---|
| Total number of labeled cells in hindbrainb | 205.9 ± 10.4 (11) | 111.1 ± 15.5 (14) | 0.54 |
| Total number of labeled cells in r2 and r3
(nV neurons: Differentiate ≈18 hpf) |
70.8 ± 7.8 (11) | 51.5 ± 5.3 (14) | 0.73 |
| Total number of labeled cells in r4 to r7
(nVII neurons: Differentiate ≈15 hpf) |
135.1 ± 8.6 (11) | 59.1 ± 13.5 (14) | 0.44 |
| Number of labeled cells in ventral spinal cordc
(Differentiate ≈ 24 hpf) |
24.1 ± 1.6 (6) | 12.9 ± 1.5 (6) | 0.54 |
| Number of Rohon–Beard cellsd
(Differentiate ≈10 hpf) |
4.7 ± 0.4 (6) | 9.8 ± 0.9 (6) | 2.1 |
Embryos were scored at 36 hours postfertilization (hpf). Numbers in parentheses represent number of embryos.
The number corresponds to the total number of labeled cells (nV, nVII) in rhombomeres 2–7.
Labeled cells (motor neurons and interneurons) were counted on one side in the ventral spinal cord in three contiguous segments at the level of the tip of the yolk tube. The number shown corresponds to the number of cells per hemisegment.
Rohon–Beard cells, which are very darkly labeled cells in the dorsal-most neural tube, were counted on one side of the spinal cord in three contiguous segments at the level of the tip of the yolk tube. The number shown corresponds to the number of cells per hemisegment.