Table 3.

Gli2 and Gli3 contribute to spinal motor neuron induction^@

Morpholino	Number of islet-labeled cells in the hindbrain		Number of islet-labeled cells in ventral spinal cord#
	Wild-type (gli1+)*	dtr mutant (gli1−/−)*	Wild-type (gli1+)*	dtr mutant (gli1−/−)*
none	294.8 ± 14.6 (6)	16.7 ± 10 (6)	22.3 ± 2.3 (6)	20.4 ± 0.6 (6)
gli2 MO§	294 ± 25.4 (6)	5.2 ± 2.8 (6)	22.7 ± 2.1 (6)	15.6 ± 1.2 (6)
	P > 0.05 (NS)	P > 0.05 (NS)	P > 0.05 (NS)	P < 0.001
none	287.5 ± 22 (4)	12 ± 4.5 (4)	35.2 ± 3.4 (4)	38.9 ± 4.5 (4)
gli3 MO£	214.5 ± 5.3 (4)	14.8 ± 3.3 (4)	16.2 ± 2.6 (4)	13.6 ± 2.9 (4)
	P < 0.001	P > 0.05 (NS)	P < 0.001	P < 0.001

^@While the numbers of control and MO-injected embryos were much higher (>40 for each treatment), we counted motor neuron cell bodies in 4–6 representative embryos for each condition. Mutant embryos were identified by the severe loss of hindbrain motor neurons.

^#Number of labeled cells per hemisegment.

^*Number of embryos scored in parenthesis.

^§Approximately 10 ng of gli2 MO (see Materials and methods) was injected per embryo based upon previous studies (Karlstrom et al., 2003).

^£Approximately 30 ng of gli3 MO was injected per embryo. In our hands, this high dose was needed to generate the ectopic fkd4 expression phenotype described for gli3 morphants (Tyurina et al., 2005). No non-specific effects were seen, and >90% of injected embryos survived and were healthy when fixed at 36 hpf.