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Frozen sections of muscle biopsies were co-stained with TUNEL (red), antibodies against laminin (violet), caspase 3 (green) and counterstained with DAPI (blue). TUNEL co-localized with DAPI indicating nuclear death. Caspase 3 and TUNEL double positive cells were present within the laminin layer of the skeletal muscle fibers. Arrows indicate an example of an apoptotic myonucleus.
