Table 1. Summary of diffraction data statistics for native gp26 crystallized at low pH and MBP-gp26 chimera crystallized at neutral and alkaline pH.
Values in parentheses are for the highest resolution shell.
| Data set | Native-gp26, pH 4.6 | MBP-gp26, pH 7.0 | MBP-gp26, pH 10.0 |
|---|---|---|---|
| Crystallization conditions | 10% PEG 8K, 0.1 M sodium acetate pH 4.6 | 40% PEG 1K, 0.1 M MOPS pH 7.0 | 40% PEG 4K, 0.1 M CAPS pH 10.0 |
| Beamline | SSRL 9-2 | BNLS X6A | BNLS X6A |
| Space group | P21 | P32 | P32 |
| Reflections (total/unique) | 870135/71223 | 338926/32511 | 310479/19166 |
| Unit-cell parameters | |||
| a (Å) | 9.97 | 43.85 | 43.20 |
| b (Å) | 14.52 | 43.85 | 43.20 |
| c (Å) | 71.17 | 272.31 | 271.23 |
| α (°) | 90.0 | 90.0 | 90.0 |
| β (°) | 90.5 | 90.0 | 120.0 |
| γ (°) | 90.0 | 90.0 | 120.0 |
| Resolution (Å) | 40–2.2 | 30–2.0 | 40–2.5 |
| Completeness (%) | 90.8 (65.4) | 95.2 (70.4) | 96.9 (84.6) |
| Rmerge (%) | 9.8 (18.4) | 8.4 (20.6) | 9.9 (24.2) |
| 〈I〉/〈σ(I)〉 | 17.2 (5.6) | 163 (4.3) | 13.6 (4.4) |