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Deficient Msg5 phosphatase activity in either the nucleus or cytoplasm confers increased sensitivity to pheromone. Halo tests were performed as described in [83]. (A)Wildtype cells; (B) GAL-MSG5 cells; (C) NES-MSG5M45A/NLS-MSG5M45A cells; (D)NES-MSG5M45A/NLS-MSG5M45AC319A; (E) NES-MSG5M45AC319A/NLS-MSG5M45A cells.
