Table 1.
- | + a | ρ value b | ρ value c | |
control (Fus3-GFP) | 1.6 ± 0.19 | 2.2 ± 0.14 | n/a | n/a |
Dig1Δ Fus3-GFP | 1.7 ± 0.17 | 1.8 ± 0.12 | 0.3323 | <0.0001 |
Dig2Δ Fus3-GFP | 1.6 ± 0.13 | 1.9 ± 0.16 | 0.4017 | <0.0001 |
control (Fus3T180AY182A-GFP) | 1.6 ± 0.18 | 2.0 ± 0.14 | n/a | n/a |
dig1Δ Fus3T180AY182A-GFP | 1.5 ± 0.16 | 2.0 ± 0.22 | 0.2722 | 0.1771 |
dig2Δ Fus3T180AY182A-GFP | 1.5 ± 0.15 | 1.9 ± 0.16 | 0.0744 | 0.3527 |
control (empty vector) | 1.6 ± 0.19 | 2.2 ± 0.14 | n/a | n/a |
Msg5 overexpression | 1.4 ± 0.13 | 1.4 ± 0.20 | 0.0001 | <0.0001 |
Msg5M45A C319A overexpression | 1.4 ± 0.12 | 1.7 ± 0.14 | <0.0001 | <0.0001 |
control (NLS-Msg5M45A/NES- | 1.7 ± 0.17 | 1.9 ± 0.20 | n/a | n/a |
Msg5M45A) | ||||
NLS-Msg5M45A C319A/NES-Msg5M45A | 1.7 ± 0.18 | 2.1 ± 0.23 | 0.8380 | 0.0003 |
NLS-Msg5M45A/NES-Msg5M45A C319A | 1.6 ± 0.18 | 2.1 ± 0.20 | 0.0490 | 0.0003 |
aRNCF ± s.d. 3 h after pheromone treatment; bρ value when compared to RNCF of untreated control; cρ value when compared to RNCF of pheromone-treated control. n = 25 for all experiments. See additional file 1 for histograms used to calculate the RNCF values for Table 1.