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qRT-PCR Analysis for SP6 mRNA Expression. SYBR Green quantitative RT-PCR was utilized to analyze SP6 expression levels in control and iron-deficient rat groups. Fold induction is shown. n = 4 for primer set I and n = 2 for primer set II; p < 0.001 between control and iron-deficients for SP6 primer set I and p = 0.01 for primer set 2. The fold changes detected with primer set I versus primer set II are not different (p = 0.78).
