Skip to main content
. Author manuscript; available in PMC: 2009 Jan 1.
Published in final edited form as: Gene Expr Patterns. 2007 Oct 9;8(2):107–116. doi: 10.1016/j.modgep.2007.09.005

Figure 2.

Figure 2

Sfmbt2 is expressed from the paternal allele in early embryos and extraembryonic tissues. (a) Genomic DNA (gDNA), or cDNA derived from Domesticus X Castaneus (DC) F1 e3.5 blastocysys (blast), e7.5 embryonic ectoderm (ee), e7.5 extraembryonic ectoderm (eee) or e7.5 ectoplacental cone (epc) was amplified with Sfmbt2SNP4103 forward and reverse primers (Table 2). This generates a 340 bp fragment from the 3′ UTR. Digestion of this PCR product with SnaBI (S) generates 226 and 114 bp fragments from the Domesticus allele, but does not digest the Castaneus allele. Digestion of the fragment with HinfI (H) generates 199 and 141 bp fragments from the Castaneus allele, but does not digest the Domesticus allele. b) Genomic DNA (gDNA) or cDNA from DC F1 e11.5 yolk sac (ys), placenta (pl) or brain (br) was amplified and disgested as described in a). c) cDNA from DC F1 e14.5 ys, pl, and br, and from e14.5 CD ys and pl was amplified and digested as described in a).