Table 1. Differential Expression of Imprinted Genes in Androgenetic and Gynogenetic Blastocysts.
Gene | Expressed allele | Differential (fold) | Up in Andros Down in Gynos | Up in Gynos Down in Andros |
---|---|---|---|---|
Slc38a4 | Paternal | 10.6 (1552) | yes | |
Mcts2 | Paternal | 9.2 (588) | yes | |
Sfmbt2 | Paternal (this study) | 8.6 (388) | yes | |
Plagl1 | Paternal | 6.3 (78) | yes | |
Osbpl5 | Maternal | 4.0 (16) | yes | |
H19 | Maternal | 3.4 (10.6) | yes | |
Gtl2 | Maternal | 3.0 (8.0) | yes |
Microarray data were analysed with ArrayAssist 2.0, and those genes with a minimum 4-fold (ln 2) difference between either androgenetic and biparental or gynogenetic and biparental probes were ranked according to the absolute difference between androgenetic and gynogenetic probes. Selected imprinted genes (Beechey at al., 2005) are included to illustrate the range of expression differences.