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. Author manuscript; available in PMC: 2008 Jul 15.
Published in final edited form as: J Immunol. 2007 Jul 15;179(2):1080–1087. doi: 10.4049/jimmunol.179.2.1080

FIGURE 2.

FIGURE 2

Effect of combination of cytokines and dsRNA on the up-regulation of TSLP. NHBE were incubated for 6 h (A) or 24 h (D) with 100 ng/ml IL-4, 100 ng/ml IL-13, 100 ng/ml IFN-γ, 1000 U/ml IFN-β, and 25 µg/ml dsRNA in the presence or absence of 100 ng/ml TNF-α as indicated. NHBE were incubated for 6 h (B) or 24 h (E) with 100 ng/ml IL-4, 25 µg/ml dsRNA, or a combination of IL-4 and dsRNA. C and F, NHBE were incubated for 1–48 h with medium control (◻), 100 ng/ml IL-4 plus 100 ng/ml TNF-α(◼), 25 µg/ml dsRNA (○), and 100 ng/ml IL-4 plus 25 µg/ml dsRNA (●). The level of TSLP mRNA was determined by real-time PCR (A–C). Concentrations of TSLP protein in the culture supernatant were measured by ELISA (D–F). Results shown are mean ± SEM of 4–7 independent experiments. NS, Not significant; N.D., not detectable. *, p < 0.05.