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. Author manuscript; available in PMC: 2008 Feb 1.
Published in final edited form as: J Neurochem. 2007 Nov 14;104(3):683–695. doi: 10.1111/j.1471-4159.2007.05072.x

Fig. 6.

Fig. 6

Role of amyloid precursor protein (APP) derivatives on the up-regulation of β-site amyloid precursor protein cleaving enzyme 1 (BACE1). (a) Transfection with APP695, significantly increases BACE1 expression and protein levels in APPko–mouse embryonic fibroblasts (MEFs). (b) Transfection with APP-C99, expressing the APP C-terminal domain resulting from the β-secretase cleavage restores the up-regulation of BACE1 in APPko-MEFs. (c) Transfection with APP intracellular domain (AID/AICD) 59, the APP derivative released by the γ-secretase cleavage at position 40 of the Aβ domain, has no effect on BACE1 expression and protein levels in APPko-MEFs. (d) Incubation with freshly prepared Aβ 1–42 induced a 3.5-fold increase in BACE1 mRNA in APPko–MEFs. Incubation with freshly prepared Aβ 1-40 induced a twofold increase in BACE1 mRNA in APPko–MEFs; *p < 0.05, **p < 0.02.