Skip to main content
. 1997 Feb 1;109(2):201–216. doi: 10.1085/jgp.109.2.201

Figure 3.

Figure 3

Effect of varied [DIDS] on valinomycin- induced net efflux (A and B) of K+ (circles) and Cl− (squares), and on the percent inhibition of the net effluxes (C and D) at 23°C (A and C) and at 37°C (B and D). Washed cells were suspended at 1.2% hematocrit in medium containing 1 mM KCl, 149 mM NaCl, and 10 mM HEPES buffer, pH 7.4, at either 25°C or at 37°C, and were pretreated with varied concentrations of DIDS for 30 min. Samples were removed before and 10 min after addition of 3 μM valinomycin. The centrifugally packed cells were analyzed for K+, Cl−, and hemoglobin, and the net effluxes were determined by the cold-quench method (see materials and methods and Freedman et al., 1994).