Table I.
Initial Cell Potassium and Hemoglobin Concentrations, Water Content, Donnan Ratio and Membrane Potential, and Constant Field K+ and Cl− Permeabilities of Human Red Blood Cells Treated with Valinomycin or Gramicidin
| Cell potassium, [K+]c (mM) | 130 ± 4 (3%) | ||
| Hemoglobin (g/ml cell water) | 0.44 ± 0.02 (5%) | ||
| Cell water (g/g cells) | 0.662 ± 0.009 (1%) | ||
| Donnan ratio, rcl | 0.67 ± 0.02 (4%) | ||
| Membrane potential, Ecl (mV) | −10 ± 0.9 (9%) | ||
| Constant field permeabilites | PK/PCl | PCl (h −1) | |
| −DIDS | 20 | 0.49 | |
| +DIDS | 74 ± 9 | 0.13 | |
The values for cell K+, hemoglobin, water content, Donnan ratio, and membrane potential are corrected for trapped medium (see materials and methods) and are means ± SD for five donors; coefficients of variation are in parentheses. The value of PK/PCl without DIDS is from Freedman and Hoffman (1979b); the value with DIDS is from Fig. 6 C (middle line). The value of PCl with DIDS is from Fig. 6 A (lower line); the value without DIDS was deduced as described in the text.