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. 1997 Feb 1;109(2):217–228. doi: 10.1085/jgp.109.2.217

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Northern analysis of proximal tubule cell RNA. The mouse kidney PMCA1-PMCA4 PCR products were randomly primed, [³²P]dCTP-labeled, and used to probe 10 μg of mouse proximal tubule cell RNA and 2 μg of mouse kidney mRNA. For all Northern analyses, lane 1, mouse kidney mRNA; lane 2, S₁ RNA; lane 3, S₂ RNA; and lane 4, S₃ RNA. (A) PMCA1 probe hybridizes with transcripts of 7.5 and 5.5 kb in all samples; (B) PMCA2 probes hybridize with a transcript of 7.1 and 4 kb in kidney mRNA; (C) PMCA3 probes hybridize with transcripts of 7.5 and 5.1 kb in mouse brain mRNA (lane 5); and (D) PMCA4 probes detect transcripts of 8.5 and 7.5 kb in all samples. Transcripts detected are appropriately sized for each PMCA isoform.