Table I.

Primers Used for Amplification of Transcripts By RT-PCR

Primer		Sequence		Reference		Annealing Temp. (°C)
PMCA1 Forward		5′-TGGCAAACAACTCAGTTGCATATAGTGG-3′		(Meszaros and Karin, 1993)		65
PMCA1 Reverse		5′-TCCTGTTCAATTCGACTCTGCAAGCCTCG-3′		(Meszaros and Karin, 1993)
PMCA2 Forward		5′-TCTGGTGAGGGTGTACTGAGGACA-3′		(Abramowitz et al., 1995)		62
PCMA2 Reverse		5′-GAGCGTCACGTCCTGTAGTGC-3′		(Abramowitz et al., 1995)
PMCA3 Forward		5′-GAAGACCTCACCCACAGAGG-3′		(Stauffer et al., 1993)		60
PMCA3 Reverse		5′-TCTGCTCCTGCTCAATTCGG-3′		(Stauffer et al., 1993)
PMCA4 Forward		5′-AAGAAGATGATGAAGGACAACAAC-3′		(Abramowitz et al., 1995)		65
PMCA4 Reverse		5′-GTTGCGTACCATATTGTCTCGGTC-3′		(Abramowitz et al, 1995)
β-Actin Forward		5′-AACCGCGAGAAGATGACCCAGATCATGTTT-3′		(Nakajima-Iijima et al., 1985)		55
β-Actin Reverse		5′-AGCAGCCGTGGCCATCTCTTGCTCGAAGTC-3′		(Nakajima-Iijima et al., 1985)
NCX1 Forward		5′-ATGCTGGGTCTGATTATGAGT-3′		(White et al., 1996a )		60
NCX1 Reverse		5′-AGTGGCTGCTTGTCATCGTA-3′		(White et al., 1996a )

The sequences of the oligodeoxynucleotide primers (listed from 5′ to 3′) used for RT-PCR analysis are shown. The optimized annealing temperature for the amplification of mouse transcripts by each primer pair is shown in the right hand column in the same row as the forward primer. The references in which the primer sequences were originally published are also listed.