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. Author manuscript; available in PMC: 2009 Jan 14.
Published in final edited form as: Toxicology. 2007 Oct 10;243(1-2):164–176. doi: 10.1016/j.tox.2007.10.004

Fig. 5.

Fig. 5

The impact of NAC pretreatment on the redox status of Trx1. A: Redox western blot of Trx1 in HMEC-1 cells treated with the indicated concentrations of acrolein for 30 min. Prior to acrolein exposure, cells were either untreated (left) or pre-treated with 2 mM NAC for 2 hr in cell culture medium with FBS and supplements. The cells were then washed twice with HBSS and then treated with acrolein. Note that NAC-pretreated cells were not tested at 1 μM acrolein. B, C, D: The percentage of total Trx1 that is in the reduced (B), partially oxidized (C), or fully oxidized (D) form in cells with or without NAC pretreatment, as determined by densitometry of the redox blots (mean ± S.D. for 3 independent experiments). ** (p < 0.01) or *** (p <0.001) relative to the corresponding “No NAC” samples.