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. 1999 Jul 6;96(14):8150–8155. doi: 10.1073/pnas.96.14.8150

Figure 5.

Figure 5

Effect of the C609T substitution on induction of NQO1 by HQ in human bone marrow cells. (A) NQO1 activity was measured after bone marrow cells C/C (●), C/T (■), or T/T (▴) at position 609 in the coding region of NQO1 were treated with 0–100 μM HQ for 24 h. Activities are reported as nmol 2,6-dichlorophenol-indophenol reduced/min per mg cellular protein. Data are presented as mean ± SD (n ≥ 3). ∗, Significantly greater than control (P ≤ 0.05). (B) NQO1 protein was measured by immunoblot analysis. Representative immunoblots of NQO1 protein in HQ-treated cells with the C/C, C/T, or T/T genotype are shown (n ≥ 3 for each genotype). Purified recombinant human NQO1 protein (5 ng) was included as a standard (NQO1). Freshly isolated bone marrow cells (F) lacked detectable NQO1 protein in all cases.