Figure 7.

Induction of NQO1 in human bone marrow mononuclear cells enriched for CD34 expression. Isolated CD34⁺ cells were cultured with or without HQ (10 μM) for 18 h. All three donors were genotyped as C/C at position 609 of the coding region of NQO1. (A) NQO1 activity. Data are presented as mean ± SD (n = 3). ∗, Significantly greater than control (P ≤ 0.05). (B) NQO1 protein was measured by immunoblot analysis. Purified recombinant human NQO1 was included as a standard (0.5 ng, NQO1). Immunoblot is representative of three separate experiments. No NQO1 activity or protein was detected in freshly isolated CD34⁺ cells (F).