Table 2.

The yields of parent, D199A mutant, and rescued viruses from quiescent and serum-starved human lung fibroblasts harvested at 5 or 20 hr after infection

HLF cells	Hr after infection	Yield*
		HSV-1(F)	R7914	R7915
Serum starved	5	1.2  ×  104	2.4  ×  104	1.3  ×  104
	20	6.4  ×  107	4.0  ×  106	5.6  ×  107
Quiescent	5	6.1  ×  104	5.5  ×  104	4.1  ×  104
	20	1.8  ×  108	1.1  ×  107	1.7  ×  108

^*Diploid human lung fibroblasts (HLF) grown in 25-cm² flasks were either serum-starved in medium containing 0.25% FCS for 5 days or allowed to become fully contact inhibited (quiescent) and maintained in same medium for 7 days. The cells were infected (0.05 PFU/cell) in the same medium in which they were maintained before infection.