Figure 1.

Recovery of L. pneumophila (log₁₀ cfu/ml) from guinea pig organs. (A) Recovery from lungs of strain AA100jm and a pool of mutant clones (pool 36) for 11 guinea pigs. Animals 1–6 received both AA100jm and pool 36, and animals 7–12 received only pool 36 bacteria. All animals except 6 and 12 were killed 2 days after infection; 6 and 12 were killed 3 days after infection. Delivered bacterial inocula are shown by the clear bar (AA100jm) and the light cross hatched bar (pool 36); the L. pneumophila lung concentrations are shown by the heavy cross hatched (AA100jm) and black bars (pool 36); e.g., for animal 1, the bars from left to right are, respectively, AA100jm input, pool 36 input, AA100 output, and pool 36 output. (B) Results for animals inoculated with the salt tolerant mutant AP2.B5 (animals 1 and 2), or both AA100jm and AP2.B5 (animals 3–6). AA100 input and output is designated by the clear bars, and AP2.B5 is designated by the cross hatched bars. L, lung; S, spleen. Animals 5 and 6 were killed 3 days after infection, and animals 1–4 were killed killed 2 days after infection. (C) Results for animals inoculated with the mip⁻ mutant AP3 (animals 1 and 2) or with AP3 and AA100jm (animals 3 and 4). AA100 input and output is designated by the clear bars, and APB3 is designated by the cross hatched bars. All animals were killed 2 days after infection.