Table 1.

Characterization of clones

Clone	Transposon insertion site	Mφ growth*		Animal model†	GenBank no.	Identity/Similarity, e value‡
		2 days	3 days
6b	L. pneumophila icmX	−3.7	−4.9	LS	3602929	86/97, e−59
3c	L. pneumophila dotB	−3.6	−5.5	LS	AF026533	100/100, e−10
2f	L. pneumophila dotO/icmB	−3.1	−4.5	LS	AF026534	100/100, e−75
6c§	L. pneumophila icmX	−4.0	−5.2	LS	3602929	91/94, e−117
8a	L. pneumophila dotF/icmG	−1.1	0.2	LS	AF026534	97/97, e−14
8g	NH¶ (608 bp upstream of L. pneumophila icmF)	−2.3	−2.1	LS
5b	L. pneumophila proA	−1.3	−0.2	LS	M31884	100/100, e−46
4a	E. coli phosphoenolpyruvate phosphotransferase (pts) homolog	−1.8	−0.5	LS	1255724	40/61, e−36
3h‖	E. coli phosphoenolpyruvate phosphotransferase (pts) homolog	−1.7	−0.5	LS	1255724	45/67, e−31
4b	Haemophilus influenzae 3-dehydroquinate synthetase (aroB) homolog	−2.9	−2.7	LS	1073786	57/72, e−71
5f**	H. influenzae 3-dehydroquinate synthetase (aroB) homolog	−1.5	−0.2	LS	1073786	34/51, e−10
4d	Quenine tRNA-ribosyltranferase (tgt) homolog	−0.5	−0.1	LS	Q55983	42/58, e−77
4c	Helicobacter pylori β-alanine synthetase homolog	−0.2	0.2	LS	2313883	46/57, e−16
8b	Hypothetical 107-kDa protein in argR-cafA region homolog	−0.3	0.0	ls	2851641	28/51, e−6
3b	NH	−0.8	0.1	LS
1e	NH	−0.3	0.1	LS

^*Difference (log₁₀ cfu/ml) between concentration of clone and parent strain grown in guinea pig alveolar macrophages, after 2 and 3 days. Data represent mean of two independent experiments. The median differences and 95% Cls between the two experiments for each strain were log₁₀ cfu/ml 0.09 (0.07–0.19), 0.26 (0.16–0.35), and 0.34 (0.22–0.46) for days 0, 2, and 3, respectively. 

^†Organs not containing detectable clone by colony blot. LS, missing from all three lungs and spleens from three animals; ls, missing from the lungs and spleens of two of three animals. 

^‡blastx search identity and similarity percent, and e value. 

^§The transposon insertion sites of clones 6b and 6c were 615 bp apart in the same direction. 

^¶NH, no homology found in blastx search of the GenBank database. 

^‖The transposon insertion sites of clones 4a and 3h were 434 bp apart and in opposite directions. 

^**The transposon insertion sites of clones 4b and 5f were 106 bp apart and in opposite directions.