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. 1978 Mar;133(3):1113–1125. doi: 10.1128/jb.133.3.1113-1125.1978

Chromosome mapping in Pseudomonas aeruginosa PAT.

J M Watson, B W Holloway
PMCID: PMC222141  PMID: 417059

Abstract

A linkage map of Pseudomonas aeruginosa PAT has been derived from the results of conjugation experiments using the plasmids FP2-2, R68, R91-5, and R68.45. FP2-2 and R68 each mobilize the chromosome from single, distinct transfer origins. R91-5 appears to mobilize the chromosome from two such origins, and R68.45 utilizes a number of transfer origins. R68 and R91-5 have both been shown to mobilize the chromosome with a polarity opposite to that by FP2-2. The locations of the transfer origins of these plasmids are such that it has not been possible to demonstrate chromosomal circularity by means of interrupted mating experiments. However, the available time-of-entry data combined with linkage data from plate mating experiments support the conclusion that the chromosome of P. aeruginosa is circular.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Achtman M. Genetics of the F sex factor in enterobacteriaceae. Curr Top Microbiol Immunol. 1973;60:79–123. doi: 10.1007/978-3-642-65502-9_3. [DOI] [PubMed] [Google Scholar]
  2. Carey K. E., Krishnapillai V. Location of prophage H90 on the chromosome of Pseudomonas aeruginosa strain PAO. Genet Res. 1974 Apr;23(2):155–164. doi: 10.1017/s0016672300014774. [DOI] [PubMed] [Google Scholar]
  3. Chandler P. M., Krishnapillai V. Characterization of Pseudomonas aeruginosa derepressed R-plasmids. J Bacteriol. 1977 May;130(2):596–603. doi: 10.1128/jb.130.2.596-603.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chandler P. M., Krishnapillai V. Phenotypic properties of R factors of Pseudomonas aeruginosa: R factors readily transferable between Pseudomonas and the Enterobacteriaceae. Genet Res. 1974 Jun;23(3):239–250. doi: 10.1017/s0016672300014890. [DOI] [PubMed] [Google Scholar]
  5. Chandler P. M., Krishnapillai V. Phenotypic properties of R factors of Pseudomonas aeruginosa: R factors transferable only in Pseudomonas aeruginosa. Genet Res. 1974 Jun;23(3):251–257. doi: 10.1017/s0016672300014907. [DOI] [PubMed] [Google Scholar]
  6. FARGIE B., HOLLOWAY B. W. ABSENCE OF CLUSTERING OF FUNCTIONALLY RELATED GENES IN PSEUDOMONAS AERUGINOSA. Genet Res. 1965 Jul;6:284–299. doi: 10.1017/s0016672300004158. [DOI] [PubMed] [Google Scholar]
  7. Haas D., Holloway B. W. R factor variants with enhanced sex factor activity in Pseudomonas aeruginosa. Mol Gen Genet. 1976 Mar 30;144(3):243–251. doi: 10.1007/BF00341722. [DOI] [PubMed] [Google Scholar]
  8. Haas D., Holloway B. W., Schamböck A., Leisinger T. The genetic organization of arginine biosynthesis in Pseudomonas aeruginosa. Mol Gen Genet. 1977 Jul 7;154(1):7–22. doi: 10.1007/BF00265571. [DOI] [PubMed] [Google Scholar]
  9. Hane M. W., Wood T. H. Escherichia coli K-12 mutants resistant to nalidixic acid: genetic mapping and dominance studies. J Bacteriol. 1969 Jul;99(1):238–241. doi: 10.1128/jb.99.1.238-241.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Isaac J. H., Holloway B. W. Control of pyrimidine biosynthesis in Pseudomonas aeruginosa. J Bacteriol. 1968 Nov;96(5):1732–1741. doi: 10.1128/jb.96.5.1732-1741.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Krishnapillai V. A novel transducing phage. Its role in recognition of a possible new host-controlled modification system in Pseudomonas aeruginosa. Mol Gen Genet. 1972;114(2):134–143. doi: 10.1007/BF00332784. [DOI] [PubMed] [Google Scholar]
  12. Loutit J. S., Marinus M. G. Investigation of the mating system of Pseudomonas aeruginosa strain 1. II. Mapping of a number of early markers. Genet Res. 1968 Aug;12(1):37–44. doi: 10.1017/s0016672300011599. [DOI] [PubMed] [Google Scholar]
  13. Low K. B. Escherichia coli K-12 F-prime factors, old and new. Bacteriol Rev. 1972 Dec;36(4):587–607. doi: 10.1128/br.36.4.587-607.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Matsumoto H., Tazaki T. FP5 factor, an undescribed sex factor of Pseudomonas aeruginosa. Jpn J Microbiol. 1973 Sep;17(5):409–417. doi: 10.1111/j.1348-0421.1973.tb00792.x. [DOI] [PubMed] [Google Scholar]
  15. Mee B. J., Lee B. T. A map order for his I, one of the genetic regions controlling histidine biosynthesis in Pseudomonas aeruginosa, using the transducing phage F116. Genetics. 1969 Jul;62(3):687–696. [PMC free article] [PubMed] [Google Scholar]
  16. Pemberton J. M., Holloway B. W. A new sex factor of Pseudomonas aeruginosa. Genet Res. 1973 Jun;21(3):263–272. doi: 10.1017/s0016672300013458. [DOI] [PubMed] [Google Scholar]
  17. Pemberton J. M., Holloway B. W. Chromosome mapping in Pseudomonas aeruginosa. Genet Res. 1972 Jun;19(3):251–260. doi: 10.1017/s0016672300014518. [DOI] [PubMed] [Google Scholar]
  18. Rolfe B., Holloway B. W. Host specificity of DNA and conjugation in Pseudomonas aeruginosa. Genetics. 1969 Feb;61(2):341–349. doi: 10.1093/genetics/61.2.341. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stanisich V. A., Bennett P. M., Richmond M. H. Characterization of a translocation unit encoding resistance to mercuric ions that occurs on a nonconjugative plasmid in Pseudomonas aeruginosa. J Bacteriol. 1977 Mar;129(3):1227–1233. doi: 10.1128/jb.129.3.1227-1233.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Stanisich V. A., Holloway B. W. A mutant sex factor of Pseudomonas aeruginosa. Genet Res. 1972 Feb;19(1):91–108. doi: 10.1017/s0016672300014294. [DOI] [PubMed] [Google Scholar]
  21. Stanisich V. A., Holloway B. W. Chromosome transfer in Pseudomonas aeruginosa mediated by R factors. Genet Res. 1971 Apr;17(2):169–172. doi: 10.1017/s0016672300012179. [DOI] [PubMed] [Google Scholar]
  22. Stanisich V., Holloway B. W. Conjugation in Pseudomonas aeruginosa. Genetics. 1969 Feb;61(2):327–339. doi: 10.1093/genetics/61.2.327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Waltho J. A. Genetic analysis of phenylalanine-responding mutants of Pseudomonas aeruginosa. J Bacteriol. 1972 Dec;112(3):1070–1075. doi: 10.1128/jb.112.3.1070-1075.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Watson J. M., Holloway B. W. Suppressor mutations in Pseudomonas aeruginosa. J Bacteriol. 1976 Mar;125(3):780–786. doi: 10.1128/jb.125.3.780-786.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Willetts N. The genetics of transmissible plasmids. Annu Rev Genet. 1972;6:257–268. doi: 10.1146/annurev.ge.06.120172.001353. [DOI] [PubMed] [Google Scholar]

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