Table 1.

Experimental constraints and structure statistics

Experimental constraints
NOE distance constraints	564
Intraresidue	331
Sequential	181
Long and medium range	52
Hydrogen bonding and planarity constraints	90
Dihedral constraints	124
Total experimental constraints	778
(Average number per nucleotide)	31.1
Structure analysis*
Average NOE violations in converged structure (>0.2 Å)	0
Angle violations in converged structures (>5°)	0
rms deviation from average structure (Å)†
Full structure	3.18 Å ± 0.56
Double-helical stem (G−1–A5; U12–C17)	0.93 Å ± 0.27
Bulge loop (C−2–A5; U12–G18)	2.60 Å ± 0.48
Loop (A5-U12)	2.93 Å ± 0.45

^*Average deviations from ideal covalent geometry are comparable to those in other publications from our laboratory (28). 

^†Calculated more than 33 converged structures of 50 calculated structures.