Figure 3.

Tissue biopsies of air pouch linings: inhibition of TNF-α-induced PMN accumulation. (A) Lining section taken 4 hr after exposure to TNF-α (20 ng/mouse) showing increased neutrophil number, low-power field inset. (B) Section taken 4 hr after exposure to TNF-α (20 ng/mouse), with prior local delivery of ATLa₂ (10 μg/mouse). (C) Section taken 4 hr after exposure to TNF-α (20 ng/mouse), with prior i.v. delivery of ATLa₂ (10 μg/mouse). (D) Section of 6-day air pouch lower lining taken from a mouse 4 hr after exposure to vehicle alone. Arrows denote neutrophils. Sections were prepared as in Materials and Methods and were stained with hematoxylin-eosin.