Figure 4.

Effects of ROS-generating and ROS-scavenging compounds on intracellular ROS levels. (A) Cells were incubated for 4 h in the presence of the ROS-generating compounds antimycin A (AA; 5 μM), H₂O₂ (1 mM), or menadione (Men.; 100 μM) before ROS determination. (B) Effect of common antioxidants on the antimycin A-dependent increase in DCF fluorescence. Cells were preincubated for 1 h with either BHA (100 μM) or flavone (+Flav.; 0.5 mM) before the addition of antimycin A (AA). DCF fluorescence was determined 4 h after the addition of antimycin A. Data are presented as percentages of the absolute values shown in A. Results represent the means (± SD) of three separate experiments.