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. 1999 Jul 6;96(14):8271–8276. doi: 10.1073/pnas.96.14.8271

Figure 5.

Figure 5

Expression of genes encoding ROS-scavenging enzymes and PR-1 in WT and Aox1 transgenic cells. RNA gel blot analysis of 25 μg of total RNA isolated from cultured tobacco cells 3–4 days after subculture. (A) Probes for ROS-scavenging transcripts were SodA and SodB from N. plumbaginifolia, GPX from N. tabacum, and SA-Cat from N. tabacum; probes were obtained by RT-PCR. (B) PR-1 from N. tabacum was obtained by RT-PCR. (C) Arabidopsis translation initiation factor elF4A was used to confirm equal loading of RNA.