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. 1999 Jul 6;96(14):8277–8282. doi: 10.1073/pnas.96.14.8277

Figure 1.

Figure 1

Thylakoid protein kinase purification by perfusion chromatography. (A) Protein elution and activity profile of protein kinase fractionation by perfusion chromatography; ○, protein concentration; ● and ■, kinase activity measured with histone S-III and isolated LHCII as substrates, respectively. (B) Presence of a protein kinase band(s) at about 65 kDa in the ammonium sulfate precipitated fraction from the crude thylakoid extract (AMS) and in the peak active fractions 20–22 of A detected by renaturation of kinase activity of SDS/PAGE resolved proteins as described in Materials and Methods; phosphorylation activity is given in nmoles 32P-mg protein−1.