Figure 3.

Effect of illumination on the phosphorylation of LHCII monomers and trimers. Isolated LHCII was phosphorylated by the solubilized kinase in the light or darkness. (A) The stained gel (SG) of the phosphorylation mixture resolved by nondenaturing SDS/PAGE into monomers (m) and trimers (t). AR, autoradiogram. (B) Quantification of the autoradiogram in A. The phosphorylation level of all samples at 10-min incubation is taken as one unit. (C) The phosphorylation mixture was subjected to denaturing SDS/PAGE that does not separate LHCII monomers from trimers, and the phosphorylation of the total LHCII band was detected after transfer to poly(vinylidene difluoride) membrane by phosphothreonine antibodies; squares and circles, trimer and monomer forms of LHCII phosphorylated in the light (open symbols) or darkness (closed symbols), respectively. a, large molecular mass aggregates; WB, Western blot.