Figure 5.

Urocortin mRNA expression is confined to the region of the Edinger–Westphal nucleus in both WT and CRH KO mouse brain. In WT animals, CRH mRNA expression (A) is clearly detectable in the PVH (vertical arrow) and amygdala (horizontal arrows). Urocortin mRNA expression is detectable in the Edinger–Westphal nucleus (B, horizontal arrow), but not in the hypothalamus or amygdala (C). In CRH KO mice, no CRH expression is detected (D), whereas the presence of urocortin mRNA in the Edinger–Westphal nucleus (E) and its absence in hypothalamus (F) are identical to those of the WT. Brain sections in C and F are adjacent to those in A and D, respectively, and thus contain the paraventricular nucleus and amygdala.